| 1. General features |
| ID #: |
| 1082 |
| Date stamp: |
| 10/3/2008 |
| Name: |
| DIGE study of effects of treatment after 24 hrs |
| Last update: |
| 2/9/2011 12:01:56 PM |
| Version: |
| 1 |
| |
| Electrophoresis type: |
| difference gel electrophoresis |
| |
| 2. Sample (1/2) |
| ID #: |
| 1467 |
| Name: |
| 2D DIGE labelling. |
| Description: |
Labelled with Cy2, Cy3 and Cy5 fluorescent labels.
Control1 Cy3 - Treatment1 Cy5 - IntStand1 Cy2
Control2 Cy3 - Treatment2 Cy5 - IntStand2 Cy2
Control3 Cy5 - Treatment3 Cy3 - IntStand3 Cy2
Control4 Cy5 - Treatment4 Cy3 - IntStand4 Cy2
Each gels samples were mixed together before being combined with rehydration buffer |
| |
| 2. Sample (2/2) |
| ID #: |
| 1468 |
| Name: |
| Picking Gel samples |
| Description: |
| 400 µg of a pooled sample made from all biological replicates of treated and untreated samples. |
| |
| 3. Protocol (1/2) |
| Name: |
| 2D pick gel protocol |
| |
| 3.1 First dimension (1/2) |
| Name: |
| First dimention IEF protocol |
| Ordinal number for this dimension: |
| 1 |
| Separation method employed: |
| isoelectric focusing (IEF) |
| |
| 3.1.1 Loading buffer
(1/2) |
| Name: |
| Rehydration buffer |
| Type: |
| rehydration buffer |
| Comments: |
| 460µl of rehydration buffer (8M urea, CHAPS and Bromophenol blue) was added to the protein pellet. |
| |
| 3.1.1.1 Buffer components |
|
chemical substance - Urea - 12(g)
|
|
chemical substance - CHAPS - 2(%)
|
|
chemical substance - Bromothymol Blue - 0.001(g)
|
|
chemical substance - Dithiothreitol (DTT) - 3.4(mg/ml)
|
|
chemical substance - Ampholyte Buffer - 5.5(microliter per milliliter)
|
| |
| 3.1.1 Loading buffer
(2/2) |
| Name: |
| Sample Buffer |
| Type: |
| sample buffer |
| Comments: |
| Equal volumes of 2 x sample buffer was added to samples. |
| |
| 3.1.1.1 Buffer components |
|
chemical substance - Urea - 7(M)
|
|
chemical substance - Thiourea - 2(M)
|
|
chemical substance - CHAPS - 2(weight-volume percentage)
|
|
chemical substance - Dithiothreitol (DTT) - 2(weight-volume percentage)
|
|
chemical substance - Ampholyte Buffer - 2(%)
|
| |
| 3.1.2 Gel matrix
|
| ID #: |
| 1320 |
| Name: |
| immobiline dry strip |
| Gel manufacture: |
| GE Healthcare |
| Physical dimensions: |
| x: 240 |
| y: 3 |
| z: 0.5 |
| Unit: millimeter |
| PH range and distribution: |
| Lower limit: 3 |
| Upper limit: 10 |
| Range type: linear distribution |
| Acrylamide concentration: |
| 4 |
| Acrylamide : Bisacrylamide ratio: |
| 4 : 3 |
| |
| 3.1.2.4 Sample application
|
| Name: |
| sample loading |
| Comments: |
| 450 µl of the rehydration buffer and sample mix was loaded into one lane of the immobiline DryStrip reswelling tray (GE healthcare). |
| |
| 3.1.3 Electrophoresis Protocol
|
| Name: |
| focusing protocol |
| Electrophoresis conditions: |
Step Voltage Time Voltage Mode
1 300 V 3 h Step
2 600 V 3 h Gradient
3 1000 V 3 h Gradient
4 8000 V 3 h Gradient
5 8000 V 4 h Step |
| |
| 3.1 Second dimension (2/2) |
| Name: |
| Second dimension |
| Ordinal number for this dimension: |
| 2 |
| Separation method employed: |
| Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), |
| |
| 3.1.2 Gel matrix
|
| ID #: |
| 1321 |
| Name: |
| polyacrylamide gel |
| Gel manufacture: |
30 % Arcylamide 188 ml
1.5 M Tris-Cl pH 8.8 113 ml
dd-H20 140 ml
10% SDS 4.5 ml
10% APS 4.5 ml Add fresh
10% TEMED 0.62 ml
|
| Physical dimensions: |
| x: 27 |
| y: 21 |
| z: 0 |
| Unit: centimeter |
| MW range and distribution: |
| Lower limit: 12000 |
| Upper limit: 120000 |
| Unit: relative molecular mass |
| Acrylamide concentration: |
| 12.5 |
| |
| 3.1.2.4 Sample application
|
| Name: |
| Sample application |
| Comments: |
| Once the gel was set the IPG strip was slotted on to the top of the gel with the acidic end to the left. It was covered with 0.5 % agarose sealing solution, before being assembled into the Ettan DALTsix System. |
| |
| 3.1.3 Electrophoresis Protocol
|
| Name: |
| Electrophoresis protocol |
| Electrophoresis conditions: |
| The system was set to run at 5W per gel for 30 min and then 4 hr at 17W per gel. |
| |
| 3.1.3.1 Running Buffer
|
| Name: |
| Running buffer |
| Type: |
| electrophoresis buffer |
| Comments: |
Reagent Amount Additional information
Tris base 30.3 g
Glycine 144 g
SDS 10 g
DD-H20 Bring to total of 1000 ml
|
| |
| 3.2 Inter-dimension Step
|
| Step name: |
| Equilibration |
| Protocol: |
| The strip was equilibrated in 10 ml SDS-equilibration buffer (2% SDS, 50mM Tris-HCL pH 8.8, 6M urea, 30% (v/v) glycerol and 0.002% (w/v) bromophenol blue) plus 100 µg DTT for 15 min with shaking. The strip was then incubated for 10 ml SDS-equilibration buffer containing 250 µg idoacetamide (IAA) for 15 min with shaking. |
| |
| 3. Protocol (2/2) |
| Name: |
| 2D DIGE protocol |
| |
| 3.1 First dimension (1/2) |
| Name: |
| First dimention IEF protocol |
| Ordinal number for this dimension: |
| 1 |
| Separation method employed: |
| isoelectric focusing (IEF) |
| |
| 3.1.1 Loading buffer
(1/2) |
| Name: |
| Rehydration buffer |
| Type: |
| rehydration buffer |
| Comments: |
| 460µl of rehydration buffer was added to the protein pellet. |
| |
| 3.1.1.1 Buffer components |
|
chemical substance - Urea - 12(g)
|
|
chemical substance - CHAPS - 2(%)
|
|
chemical substance - Bromothymol Blue - 0.001(g)
|
|
chemical substance - Dithiothreitol (DTT) - 3.4(mg/ml)
|
|
chemical substance - Ampholyte Buffer - 5.5(microliter per milliliter)
|
| |
| 3.1.1 Loading buffer
(2/2) |
| Name: |
| Sample Buffer |
| Type: |
| sample buffer |
| Comments: |
| Equal volumes of 2 x sample buffer was added to samples. |
| |
| 3.1.1.1 Buffer components |
|
chemical substance - Urea - 7(M)
|
|
chemical substance - Thiourea - 2(M)
|
|
chemical substance - CHAPS - 2(weight-volume percentage)
|
|
chemical substance - Dithiothreitol (DTT) - 2(weight-volume percentage)
|
|
chemical substance - Ampholyte Buffer - 2(%)
|
| |
| 3.1.2 Gel matrix
|
| ID #: |
| 1322 |
| Name: |
| immobiline dry strip |
| Gel manufacture: |
| GE Healthcare |
| Physical dimensions: |
| x: 240 |
| y: 3 |
| z: 0.5 |
| Unit: millimeter |
| PH range and distribution: |
| Lower limit: 3 |
| Upper limit: 10 |
| Range type: linear distribution |
| Acrylamide concentration: |
| 4 |
| Acrylamide : Bisacrylamide ratio: |
| 4 : 3 |
| |
| 3.1.2.4 Sample application
(1/2) |
| Name: |
| sample loading |
| Comments: |
| 450 µl of the rehydration buffer and sample mix was loaded into one lane of the immobiline DryStrip reswelling tray (GE healthcare). |
| |
| 3.1.2.4 Sample application
(2/2) |
| Name: |
| sample loading |
| Comments: |
| 450 µl of the rehydration buffer and sample mix was loaded into one lane of the immobiline DryStrip reswelling tray (GE healthcare). |
| |
| 3.1.3 Electrophoresis Protocol
|
| Name: |
| focusing protocol |
| Electrophoresis conditions: |
Step Voltage Time Voltage Mode
1 300 V 3 h Step
2 600 V 3 h Gradient
3 1000 V 3 h Gradient
4 8000 V 3 h Gradient
5 8000 V 4 h Step |
| |
| 3.1 Second dimension (2/2) |
| Name: |
| Second dimension |
| Ordinal number for this dimension: |
| 2 |
| Separation method employed: |
| Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) |
| |
| 3.1.2 Gel matrix
(1/4) |
| ID #: |
| 1323 |
| Name: |
| polyacrylamide gel |
| Gel manufacture: |
30 % Arcylamide 188 ml
1.5 M Tris-Cl pH 8.8 113 ml
dd-H20 140 ml
10% SDS 4.5 ml
10% TEMED 0.62 ml
APS added once solution has been refrigerated for 1 hour:
10% APS 4.5 ml Made up fresh
|
| Physical dimensions: |
| x: 27 |
| y: 21 |
| z: 0 |
| Unit: centimeter |
| MW range and distribution: |
| Lower limit: 12000 |
| Upper limit: 120000 |
| Unit: r |
| Acrylamide concentration: |
| 12.5 |
| |
| 3.1.2.4 Sample application
|
| Name: |
| Sample application |
| Comments: |
| Once the gel was set the IPG strip was slotted on to the top of the gel with the acidic end to the left. It was covered with 0.5 % agarose sealing solution, before being assembled into the Ettan DALTsix System. |
| |
| 3.1.2 Gel matrix
(2/4) |
| ID #: |
| 1324 |
| Name: |
| polyacrylamide gel |
| Gel manufacture: |
30 % Arcylamide 188 ml
1.5 M Tris-Cl pH 8.8 113 ml
dd-H20 140 ml
10% SDS 4.5 ml
10% TEMED 0.62 ml
APS added once solution has been refrigerated for 1 hour:
10% APS 4.5 ml Made up fresh
|
| Physical dimensions: |
| x: 27 |
| y: 21 |
| z: 0 |
| Unit: centimeter |
| MW range and distribution: |
| Lower limit: 12000 |
| Upper limit: 120000 |
| Unit: r |
| Acrylamide concentration: |
| 12.5 |
| |
| 3.1.2.4 Sample application
(1/2) |
| Name: |
| Sample application |
| Comments: |
| Once the gel was set the IPG strip was slotted on to the top of the gel with the acidic end to the left. It was covered with 0.5 % agarose sealing solution, before being assembled into the Ettan DALTsix System. |
| |
| 3.1.2.4 Sample application
(2/2) |
| Name: |
| Sample application |
| Comments: |
| Once the gel was set the IPG strip was slotted on to the top of the gel with the acidic end to the left. It was covered with 0.5 % agarose sealing solution, before being assembled into the Ettan DALTsix System. |
| |
| 3.1.2 Gel matrix
(3/4) |
| ID #: |
| 1325 |
| Name: |
| polyacrylamide gel |
| Gel manufacture: |
30 % Arcylamide 188 ml
1.5 M Tris-Cl pH 8.8 113 ml
dd-H20 140 ml
10% SDS 4.5 ml
10% TEMED 0.62 ml
APS added once solution has been refrigerated for 1 hour:
10% APS 4.5 ml Made up fresh
|
| Physical dimensions: |
| x: 27 |
| y: 21 |
| z: 0 |
| Unit: centimeter |
| MW range and distribution: |
| Lower limit: 12000 |
| Upper limit: 120000 |
| Unit: r |
| Acrylamide concentration: |
| 12.5 |
| |
| 3.1.2.4 Sample application
(1/2) |
| Name: |
| Sample application |
| Comments: |
| Once the gel was set the IPG strip was slotted on to the top of the gel with the acidic end to the left. It was covered with 0.5 % agarose sealing solution, before being assembled into the Ettan DALTsix System. |
| |
| 3.1.2.4 Sample application
(2/2) |
| Name: |
| Sample application |
| Comments: |
| Once the gel was set the IPG strip was slotted on to the top of the gel with the acidic end to the left. It was covered with 0.5 % agarose sealing solution, before being assembled into the Ettan DALTsix System. |
| |
| 3.1.2 Gel matrix
(4/4) |
| ID #: |
| 1326 |
| Name: |
| polyacrylamide gel |
| Gel manufacture: |
30 % Arcylamide 188 ml
1.5 M Tris-Cl pH 8.8 113 ml
dd-H20 140 ml
10% SDS 4.5 ml
10% TEMED 0.62 ml
APS added once solution has been refrigerated for 1 hour:
10% APS 4.5 ml Made up fresh
|
| Physical dimensions: |
| x: 27 |
| y: 21 |
| z: 0 |
| Unit: centimeter |
| MW range and distribution: |
| Lower limit: 12000 |
| Upper limit: 120000 |
| Unit: r |
| Acrylamide concentration: |
| 12.5 |
| |
| 3.1.2.4 Sample application
(1/2) |
| Name: |
| Sample application |
| Comments: |
| Once the gel was set the IPG strip was slotted on to the top of the gel with the acidic end to the left. It was covered with 0.5 % agarose sealing solution, before being assembled into the Ettan DALTsix System. |
| |
| 3.1.2.4 Sample application
(2/2) |
| Name: |
| Sample application |
| Comments: |
| Once the gel was set the IPG strip was slotted on to the top of the gel with the acidic end to the left. It was covered with 0.5 % agarose sealing solution, before being assembled into the Ettan DALTsix System. |
| |
| 3.1.3 Electrophoresis Protocol
|
| Name: |
| Electrophoresis protocol |
| Electrophoresis conditions: |
| The system was set to run at 5W per gel for 30 min and then 4 hr at 17W per gel. |
| |
| 3.1.3.1 Running Buffer
|
| Name: |
| Running buffer |
| Type: |
| electrophoresis buffer |
| Comments: |
Reagent Amount Additional information
Tris base 30.3 g
Glycine 144 g
SDS 10 g
DD-H20 Bring to total of 1000 ml
|
| |
| 3.2 Inter-dimension Step
|
| Step name: |
| Equilibration |
| Protocol: |
| The strip was equilibrated in 10 ml SDS-equilibration buffer (2% SDS, 50mM Tris-HCL pH 8.8, 6M urea, 30% (v/v) glycerol and 0.002% (w/v) bromophenol blue) plus 100 µg DTT for 15 min with shaking. The strip was then incubated for 10 ml SDS-equilibration buffer containing 250 µg idoacetamide (IAA) for 15 min with shaking. |
| |
| 4. Direct detection
|
| Name of direct detection process: |
| Staining |
| Protocol: |
| To visualize the proteins a SYPRO® Ruby protein gel stain (Invitrogen) was used. The gel was fixed in 40% (v/v) MeOH, 10% (v/v) acetic acid for one hour then washed twice with ddH2O for 10 min before staining with SYPRO® Ruby gel stain overnight. The gel was then washed in 10% (v/v) MeOH, 7% (v/v) acetic acid for one hour followed by two 5 min washes in ddH2O. |
| |
| 4.1 Direct detection agents |
|
SYPRO Ruby - Sufficient to cover gel
|
| |
| 6. Image Acquisition (1/5) |
| Name: |
| Pick gel scan |
| Protocol: |
Dye Excitation Filter (nm) Emission Filter (nm)
Sypro 540/25 595/25
|
| Reference to gel matrix: |
| 1321 |
| |
| 6.1 Equipment
|
| Name: |
| Scanning |
| Type of equipment: |
| scanner |
| Calibration: |
|
NO
|
| Model: |
| EttanTM DIGE Imager |
| Manufacturer: |
| //CV//MS:1000494 |
| |
| 6. Image Acquisition (2/5) |
| Name: |
| DIGE gel scan |
| Protocol: |
Dye Excitation Filter (nm) Emission Filter (nm)
Cy2 480/30 530/40
Cy3 540/25 595/25
Cy5 635/30 680/30
|
| Reference to gel matrix: |
| 1323 |
| |
| 6.1 Equipment
|
| Name: |
| Scanning |
| Type of equipment: |
| scanner |
| Calibration: |
|
NO
|
| Equipment specific parameters: |
| Default parameter settings |
| Model: |
| EttanTM DIGE Imager |
| Manufacturer: |
| //CV//MS:1000494 |
| Reference (URI): |
| http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/2d_electrophoresis~scanners~ettan_dige_imager?OpenDocument&parentid=63005642&moduleid=166483 |
| |
| 6.2 Image Acquisition Software |
| Name: |
| image acquistion software |
| Version: |
| Ettan DIGE Imager software 1.0 |
| Vendor: |
| GE Healthcare Life Sciences |
| |
| 6. Image Acquisition (3/5) |
| Name: |
| DIGE gel scan |
| Protocol: |
Dye Excitation Filter (nm) Emission Filter (nm)
Cy2 480/30 530/40
Cy3 540/25 595/25
Cy5 635/30 680/30
|
| Reference to gel matrix: |
| 1324 |
| |
| 6.1 Equipment
|
| Name: |
| Scanning |
| Type of equipment: |
| scanner |
| Calibration: |
|
NO
|
| Equipment specific parameters: |
| Default parameter settings |
| Model: |
| EttanTM DIGE Imager |
| Manufacturer: |
| //CV//MS:1000494 |
| Reference (URI): |
| http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/2d_electrophoresis~scanners~ettan_dige_imager?OpenDocument&parentid=63005642&moduleid=166483 |
| |
| 6.2 Image Acquisition Software |
| Name: |
| image acquistion software |
| Vendor: |
| GE Healthcare Life Sciences |
| |
| 6. Image Acquisition (4/5) |
| Name: |
| DIGE gel scan |
| Protocol: |
Dye Excitation Filter (nm) Emission Filter (nm)
Cy2 480/30 530/40
Cy3 540/25 595/25
Cy5 635/30 680/30
|
| Reference to gel matrix: |
| 1325 |
| |
| 6.1 Equipment
|
| Name: |
| Scanning |
| Type of equipment: |
| scanner |
| Calibration: |
|
NO
|
| Equipment specific parameters: |
| Default parameter settings |
| Model: |
| EttanTM DIGE Imager |
| Manufacturer: |
| //CV//MS:1000494 |
| Reference (URI): |
| http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/2d_electrophoresis~scanners~ettan_dige_imager?OpenDocument&parentid=63005642&moduleid=166483 |
| |
| 6.2 Image Acquisition Software |
| Name: |
| image acquistion software |
| Vendor: |
| GE Healthcare Life Sciences |
| |
| 6. Image Acquisition (5/5) |
| Name: |
| DIGE gel scan |
| Protocol: |
Dye Excitation Filter (nm) Emission Filter (nm)
Cy2 480/30 530/40
Cy3 540/25 595/25
Cy5 635/30 680/30
|
| Reference to gel matrix: |
| 1326 |
| |
| 6.1 Equipment
|
| Name: |
| Scanning |
| Type of equipment: |
| scanner |
| Calibration: |
|
NO
|
| Equipment specific parameters: |
| Default parameter settings |
| Model: |
| EttanTM DIGE Imager |
| Manufacturer: |
| //CV//MS:1000494 |
| Reference (URI): |
| http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/2d_electrophoresis~scanners~ettan_dige_imager?OpenDocument&parentid=63005642&moduleid=166483 |
| |
| 6.2 Image Acquisition Software |
| Name: |
| image acquistion software |
| Vendor: |
| GE Healthcare Life Sciences |
| |
| 7. Image (1/13) |
| Name of indirect detection process: |
| Gel1-Cy2 |
| Format: |
| Tagged Image File Format |
| Dimensions: |
| X: 2537 pixels |
| Y: 2041 pixels |
| Resolution: |
| 252 dots per inch |
| Bit-depth: |
| 16 bits |
| Stored in: |
| http://estrellapolar.cnb.csic.es/proteored/MIAPE/Ficheros/462/61713_b_STANDARD_CY2.gel |
| Reference to gel matrix: |
| 1323 |
| |
| 7. Image (2/13) |
| Name of indirect detection process: |
| Gel1-Cy3 |
| Format: |
| Tagged Image File Format |
| Dimensions: |
| X: 2537 pixels |
| Y: 2041 pixels |
| Resolution: |
| 252 dots per inch |
| Bit-depth: |
| 16 bits |
| Stored in: |
| http://estrellapolar.cnb.csic.es/proteored/MIAPE/Ficheros/462/61713_b_CY3.gel |
| Reference to gel matrix: |
| 1323 |
| |
| 7. Image (3/13) |
| Name of indirect detection process: |
| Gel1-Cy5 |
| Format: |
| Tagged Image File Format |
| Dimensions: |
| X: 2537 pixels |
| Y: 2041 pixels |
| Resolution: |
| 252 dots per inch |
| Bit-depth: |
| 16 bits |
| Stored in: |
| http://estrellapolar.cnb.csic.es/proteored/MIAPE/Ficheros/462/61713_b_CY5.gel |
| Reference to gel matrix: |
| 1323 |
| |
| 7. Image (4/13) |
| Name of indirect detection process: |
| Gel2-Cy2 |
| Format: |
| Tagged Image File Format |
| Dimensions: |
| X: 2537 pixels |
| Y: 2041 pixels |
| Resolution: |
| 252 dots per inch |
| Bit-depth: |
| 16 bits |
| Stored in: |
| http://estrellapolar.cnb.csic.es/proteored/MIAPE/Ficheros/462/61714_STANDARD_CY2.gel |
| Reference to gel matrix: |
| 1324 |
| |
| 7. Image (5/13) |
| Name of indirect detection process: |
| Gel2-Cy3 |
| Format: |
| Tagged Image File Format |
| Dimensions: |
| X: 2537 pixels |
| Y: 2041 pixels |
| Resolution: |
| 252 dots per inch |
| Bit-depth: |
| 16 bits |
| Stored in: |
| http://estrellapolar.cnb.csic.es/proteored/MIAPE/Ficheros/462/61714_CY3.gel |
| Reference to gel matrix: |
| 1324 |
| |
| 7. Image (6/13) |
| Name of indirect detection process: |
| Gel2-Cy5 |
| Format: |
| Tagged Image File Format |
| Dimensions: |
| X: 2537 pixels |
| Y: 2041 pixels |
| Resolution: |
| 252 dots per inch |
| Bit-depth: |
| 16 bits |
| Stored in: |
| http://estrellapolar.cnb.csic.es/proteored/MIAPE/Ficheros/462/61714_CY5.gel |
| Reference to gel matrix: |
| 1324 |
| |
| 7. Image (7/13) |
| Name of indirect detection process: |
| Gel3-Cy2 |
| Format: |
| Tagged Image File Format |
| Dimensions: |
| X: 2537 pixels |
| Y: 2041 pixels |
| Resolution: |
| 252 dots per inch |
| Bit-depth: |
| 16 bits |
| Stored in: |
| http://estrellapolar.cnb.csic.es/proteored/MIAPE/Ficheros/462/61715_STANDARD_CY2.gel |
| Reference to gel matrix: |
| 1325 |
| |
| 7. Image (8/13) |
| Name of indirect detection process: |
| Gel3-Cy3 |
| Format: |
| Tagged Image File Format |
| Dimensions: |
| X: 2537 pixels |
| Y: 2041 pixels |
| Resolution: |
| 252 dots per inch |
| Bit-depth: |
| 16 bits |
| Stored in: |
| http://estrellapolar.cnb.csic.es/proteored/MIAPE/Ficheros/462/61715_CY3.gel |
| Reference to gel matrix: |
| 1325 |
| |
| 7. Image (9/13) |
| Name of indirect detection process: |
| Gel3-Cy5 |
| Format: |
| Tagged Image File Format |
| Dimensions: |
| X: 2537 pixels |
| Y: 2041 pixels |
| Resolution: |
| 252 dots per inch |
| Bit-depth: |
| 16 bits |
| Stored in: |
| http://estrellapolar.cnb.csic.es/proteored/MIAPE/Ficheros/462/61715_CY5.gel |
| Reference to gel matrix: |
| 1325 |
| |
| 7. Image (10/13) |
| Name of indirect detection process: |
| Gel4-Cy2 |
| Format: |
| Tagged Image File Format |
| Dimensions: |
| X: 2537 pixels |
| Y: 2041 pixels |
| Resolution: |
| 252 dots per inch |
| Bit-depth: |
| 16 bits |
| Stored in: |
| http://estrellapolar.cnb.csic.es/proteored/MIAPE/Ficheros/462/61716_STANDARD_CY2.gel |
| Reference to gel matrix: |
| 1326 |
| |
| 7. Image (11/13) |
| Name of indirect detection process: |
| Gel4-Cy3 |
| Format: |
| Tagged Image File Format |
| Dimensions: |
| X: 2537 pixels |
| Y: 2041 pixels |
| Resolution: |
| 252 dots per inch |
| Bit-depth: |
| 16 bits |
| Stored in: |
| http://estrellapolar.cnb.csic.es/proteored/MIAPE/Ficheros/462/61716_CY3.gel |
| Reference to gel matrix: |
| 1326 |
| |
| 7. Image (12/13) |
| Name of indirect detection process: |
| Gel4-Cy5 |
| Format: |
| Tagged Image File Format |
| Dimensions: |
| X: 2537 pixels |
| Y: 2041 pixels |
| Resolution: |
| 252 dots per inch |
| Bit-depth: |
| 16 bits |
| Stored in: |
| http://estrellapolar.cnb.csic.es/proteored/MIAPE/Ficheros/462/61716_CY5.gel |
| Reference to gel matrix: |
| 1326 |
| |
| 7. Image (13/13) |
| Name of indirect detection process: |
| Pickgel |
| Format: |
| Tagged Image File Format |
| Dimensions: |
| X: 2537 pixels |
| Y: 2041 pixels |
| Resolution: |
| 252 dots per inch |
| Bit-depth: |
| 16 bits |
| Stored in: |
| http://estrellapolar.cnb.csic.es/proteored/MIAPE/Ficheros/462/PG2.jpg |
| Reference to gel matrix: |
| 1321 |
